Midlands Innovation Flow Cytometry Group - Introduction to Flow Cytometry Course

Course tutors: Shahram Golbabapour – University of Birmingham and David Onion – University of Nottingham

Programme

12:30-13:30 Session 1

• What is flow cytometry and how does it work?

• Applications of flow cytometry in biomedical research.

• What is cell sorting and how does it work?

• What is Imaging Cytometry and how does it work?

• Information / further reading / useful websites / analysis software.

13:30-13:45 Break

13:45-14:45 Session 2

• Reagents for flow cytometry: antibodies, fluorophores and more.

• Basic design of flow cytometry experiments.

• Panel design, colours that overlap and compensation.

15:45-15:00 Break

15:00-16:00 Live Flow Cytometry Demonstrations

• Operating a flow cytometer.
• Creating a protocol.
• Setting compensation.
• Running Samples